Extracellular production of designer HAAs
Production process for the preparation of designer HAAs
One important biosynthetic precursor of fatty alcohols is hydroxyalkanoyloxy alkanoic acid (HAA). HAA can be produced by microbes using sugars as substrate. Currently, no commercial production of HAA is established in the art and only few literature exist that relates to HAA production. Due to their amphiphilic nature, HAA can be used as biosurfactant on one hand and as a precursor for the synthesis of alkanes, which can be used as fuel especially aviation fuel on the other hand.
With the goal of diminishing dependency from oil and turning towards a sustainable chemical industry based on renewable resources, a demand for catalysts, which are able to provide basic chemicals for the chemical industry out of plant resources (e.g. oils or sugars) arises. One important class of said basic chemicals are fatty alcohols.
The annual production of plant oils is located in the range of 140 million tons and market increases are difficult to achieve and will be accompanied by severely negative impact on environment. In contrast, the potential of using sugars is tremendous, as about 790 million tons are easily available and further 800 million tons can be obtained from the timber industry.
Short chain fatty alcohols are rarely derived from renewable resources because the primarily used oil plants produce oils with longer fatty acids, thus hindering the production of such short chain fatty alcohols. In addition, nearly all oil plants produce a broad product spectrum with the result that a specific production of a narrowly defined fat alcohol composition or even a single fatty alcohol is hampered and is thus connected with high costs. Furthermore, these products derived from plants or yeast cells are accumulated intracellular, with consequently extensive down-stream processes.
The present invention provides a novel procedure for a selective and quantitative (> 1g/L) production of HAAs from biotechnological process engineering.
Industrial relevant strains like P. putida and E. coli have been tested and specific chain lengths of HAAs (short or long) can be obtained via direct product secretion into the media. Within one day, already 40% of theoretical product (in view of glucose as C-source) can be achieved.
- Effective production process of HAAs
- Extracellular product accumulation is equivalent to easy separation from biological processes
- Different short and / or long side chains (C8, C10, C12, C14, C16) can be provided
- Very fast production times within one day P. putida KT2440 generally regarded as safe
The described technology enables the fast and competitive production of specific HAAs via biotechnological process technology.
On behalf of the University of Aachen, PROvendis offers access to rights for commercial use as well as the opportunity for further co-development. In case of interest we are pleased to inform you about the current patent status.
StichworteHAA, hydroxyalkanoyloxy alkanoic acid, biotechnology process development, Hydroxyalkanoylalkansäure, Biotechnologische Prozesse, P. putida KT2440, biosynthetic precursor, commercial production, fatty alcohol, E. coli, RWTH, platform Technology, Research, PROvendis