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Gfi1 P2A/P2A knock-in mouse model

Ref-Nr: TA-3947


Kurzfassung

A model to study defects in hematopoiesis and inner ear development


Hintergrund

The Snail/Gfi1 (SNAG) family of zinc finger proteins is a group of transcriptional repressors. Gfi1 is expressed in the hematopoietic and nervous system. Consequently, mutations of Gfi1 cause defects in hematopoiesis and inner ear development.


Lösung

In the Gfi1P2A/P2A mouse strain, a point mutation has been inserted in the SNAG domain that replaces a proline at amino acid position 2 by alanine (P2A). This completely abrogates the activity of Gfi1 as transcriptional repressor.

Gfi1 and its paralogue Gfi1b have overlapping, however differential functions in hematopoiesis. Loss of Gfi1 in mice affects pre-T-cell differentiation, the development of neutrophil granulocytes and inner ear hair cells, whereas in contrast loss of Gfi1b impairs the development of erythroid cells and megacaryocytes. Therefore, Gfi1P2A/P2A mice can be used as a model to study and treat deafness as a consequence of defects of inner ear development as well as defects of hematopoiesis in immunological disorders.


Vorteile

In vivo model with significant defects of inner ear development and hematopoiesis:

  • study deafness
  • develop new approaches to treat deafness
  • study immunological disorders

Service

On behalf of the University of Duisburg-Essen, PROvendis offers access to the mouse model under a Material License Agreement.


PROvendis GmbH

Dipl.-Biol. Kordula Kruber
+49.208 94105-30
kk@provendis.info
www.provendis.info
Adresse
Schloßstr. 11-15
45468 Mülheim an der Ruhr



Entwicklungsstand

Funktionsnachweis


Stichworte

SNAG, Zinkfinger-Proteine, Epxressionsrepressoren, Entwicklung des Innenohrs, Taubheit, Hämatopoese SNAG, zinc finger proteins, expression repressors, inner ear development, deafness, hematopoiesis

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