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Tmem16f-deficient mice

Ref-Nr: TA-3481


Kurzfassung

A model for studying osteoporosis


Hintergrund

During vertebrate skeletal development, osteoblasts produce a mineralized bone matrix by deposition of hydroxyapatite crystals in the extracellular matrix. Anoctamin6/Tmem16F (Ano6) belongs to a conserved family of transmembrane proteins. The gene Ano6 encodes a Ca-activated chloride channel. In addition, Ano6 has been linked to phosphatidylserine (PS) scrambling in the plasma membrane. During skeletogenesis, Ano6 mRNA is expressed in differentiating and mature osteoblasts.


Lösung

Tmem16f-deficient mice were generated by complete gene deletion. Deletion of Ano6 in mice results in reduced skeleton size and skeletal deformities. However, analysis of endochondral ossification on the molecular level reveals that proliferation and differentiation of chondrocytes is not affected, whereas the ossification process is significantly delayed. The transgenic mice display increased regions of nonmineralized, Ibsp-expressing osteoblasts in the periosteum during embryonic development and increased areas of uncalcified osteoid postnatally. Furthermore, calcium-dependent phospholipid scrambling is impaired in Tmem16f-defcient osteoblasts, supporting a function of PS exposure in the deposition of hydroxyapatite.


Vorteile

  • Mouse model with significantly delayed ossification and skeletal deformities
  • Valuable model for osteoporosis and others

Service

On behalf of the University of Duisburg-Essen, PROvendis offers access to the mouse model under a Material License Agreement.


PROvendis GmbH

Dr. Jürgen Walkenhorst
+49.208 94105-25
jw@provendis.info
www.provendis.info
Adresse
Schloßstr. 11-15
45468 Mülheim an der Ruhr



Entwicklungsstand

Funktionsnachweis


Stichworte

Maus, mouse, Mausmodell, mouse model, complete gene deletion, Forschungstool, research tool, Osteoporose, osteoporosis, Skelettentwicklung, skeletal development, ossification, TMEM16F, Ano6, Anoctamin-6, Kalzium aktivierter Chlorid-Kanal, Ca activated chlorid channel

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