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Microfluidic wound healing & migration assay: automated, miniaturized and integrated screening platform

Ref-Nr: TA-M059/2015


Kurzfassung

We have developed a method capable of mechanically inducing circular cell-free areas within confluent cell layers using a pneumatically-controlled membrane deflection/compressi on method.


Hintergrund

Despite the wide-spread use of migration and wound healing assays, to date no satisfactory technological solutions are available that allow for the automated, miniaturized and integrated induction of highly reproducible wounds. We therefore developed a lab-on-a-chip capable of mechanically inducing circular cell-free areas within confluent cell layers using a pneumatically-controlled membrane deflection/compression method.


Bilder & Videos


Lösung

1. Creation of high reproducibility circular cell-free areas using cell depletion approach
2. Creation of high reproducibility circular cell-free areas using cell exclusion approach
3. Integration of planar pneumatically-actuated membranes
4. Repeatable mechanical wounding of the same area without removal of ECM coating
5. Limited number of  injured cells along the edge of the cell-free area
6. Removal of cell debris during wound induction
7. Fully automatable, miniaturized and integrated membrane deflection/compression method with controlled pressure profiles


Anwendungsbereiche

  • Toxicity tests
  • ADME studies
  • Compound screening
  • Drug development

Wissenstransferzentrum Ost (WTZ Ost)

DI Dr. Hildegard Sieberth
+43 1 58801 415243
hildegard.sieberth@tuwien.ac.at
www.wtz-ost.at
Adresse
Karlsplatz 13
1040 Wien



Entwicklungsstand

Machbarkeit


Stichworte

Wundheilung, Zellmigrationstest, Zellausschluss Methode, Zellabtötungsmethode, Mechanisches Stempeln, Mikrofluidik

Angebot Anbieter-Website


Kontakt | Geschäftsstelle

TechnologieAllianz e. V.
Christiane Bach-Kaienburg
(Geschäftsstellenleiterin)

c/o PROvendis GmbH
Schloßstr. 11-15
D-45468 Mülheim an der Ruhr