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Pipette device for delicate transfer of tissue in immunohistochemistry staining procedures

Ref-Nr: TA-P-1295


Kurzfassung

Staining of tissue is a standard method in biological laboratories, but also in clinical environment for diagnostics such as immunohistochemistry (IHC). After a certain incubation period the protocol often requires the transfer of the tissue into another staining chamber. This transfer is a critical step since the thickness of the paraffin embedded slices is smaller than 10 micrometers. As a technical solution a disposable pipette is engineered to have a hole at a specified spot for pressure regulation. This ensures that no under pressure occurs, which might be able to damage the tissue mechanically and/or inhibits the tissue from being sucked into the pipette.


Hintergrund

Staining of tissue is a standard method in biological laboratories, but also in clinical environment for diagnostics such as immunohistochemistry (IHC). After a certain incubation period the protocol often requires the transfer of the tissue into another staining chamber. This transfer is a critical step since the thickness of the paraffin embedded slices is smaller than 10 micrometers and, therefore, not very resistant against mechanical force so it bears the danger to destroy the biological material. In addition, the slice can be easily sucked into the laboratory pipette during transferring procedure.

As a technical solution a disposable pipette is engineered to have a hole at a specified spot for pressure regulation. This ensures that no under pressure occurs, which might be able to damage the tissue mechanically and/or inhibits the tissue from being sucked into the pipette. 


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Lösung

 a disposable pipette with a hole at a specified spot for pressure regulation


Vorteile

  • Disposable pipette device for transfer of tissue such as in immunohistochemistry (IHC) 
  • Applicable for all commercial available pipette suction devices
  • In particular useful for paraffin embedded slices smaller than 10 micrometer

Anwendungsbereiche

  • The prototype of the device has been optimized for different experiments and has already been demonstrated to be successful in staining procedures of IHC.
  • DKFZ is looking for a commercial partner to produce and distribute the device for biological and medicinal laboratories.

Deutsches Krebsforschungszentrum DKFZ

Dr. Frieder Kern
+49-6221-42-2952
f.kern@dkfz.de
www.dkfz.de
Adresse
Im Neuenheimer Feld 280
69120 Heidelberg



Entwicklungsstand

Prototyp


Stichworte

Research Tools, Devices, Microscopy

Angebot Anbieter-Website


Kontakt | Geschäftsstelle

TechnologieAllianz e. V.
Christiane Bach-Kaienburg
(Geschäftsstellenleiterin)

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D-45468 Mülheim an der Ruhr