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Monoclonal antibody against SUMO1


Kurzfassung

The antibody of this invention is a mouse monoclonal antibody directed against SUMO176-86 recognizing SUMO1 of human, mouse, chicken and X. laevis. It is suitable for western blot and immune precipitation as well as for large-scale enrichment of SUMOylated species by IP / peptide elution.


Hintergrund

SUMOylation is an essential modification that regulates hundreds of proteins in eukaryotic cells. The current invention provides an antibody which targets SUMO1 protein of a wide variety of different species.


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Lösung

  • Monoclonal mouse antibody directed against SUMO1 76-86.
  • Antibody recognizes SUMO1 of human, mouse, chicken and X. laevis.

Vorteile

  • Suitable for western blot and immune precipitation
  • Suitable for large-scale enrichment of SUMOylated species by IP / peptide elution

Anwendungsbereiche

The antibody recognizes an epitope that is shared in SUMO1 proteins of human, mouse, chicken and X. laevis. The antibody is especially useful for the enrichment of sumoylated species from cells and tissues, as it immunoprecipitates efficiently upon denaturing lysis. Bound proteins can be efficiently removed form solid phase bound antibodies by elution with epitope spanning peptides (see Becker et. al. Nat Struct Mol Biol. 2013 Apr;20(4):525-31; Barysch et al. Nature Protocols 2014, 9:896-909)


Deutsches Krebsforschungszentrum DKFZ

Dr. Frieder Kern
+49-6221-42-2952
f.kern@dkfz.de
www.dkfz.de
Adresse
Im Neuenheimer Feld 280
69120 Heidelberg



Entwicklungsstand

Prototyp


Stichworte

Therapeutics, Research Tools

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Kontakt | Geschäftsstelle

TechnologieAllianz e. V.
Christiane Bach-Kaienburg
(Geschäftsstellenleiterin)

c/o PROvendis GmbH
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D-45468 Mülheim an der Ruhr