Improved Version of The Chorionallantoic Membrane (CAM) Assay
The invention discloses an improved version of the chorionallantic membrane, for accurate quantification of metastatic cells in different organs of the chicken embryo at a high sensitivity.
The chorionallantic membrane assay, is a wellestablished in vivo assay for simulation of intravasation, invasion and metastasis of tumor cells. Basically tumor cells are placed in the space outside the chorionallantic membrane of a fertilized chicken egg at day 10 p.c. After different time points the presence of tumor cells inside the chicken embryo or its germinal membranes or vessels can be used to determine the invasive and metastatic capacity of the used tumor cells.
The offered method is based on the standard CAM assay and uses TaqMan probes in combination with real-time reverse transcriptase technology for accurate quantification of metastatic cells in different organs of the chicken embryo [see Figure]. Even it is an in vivo test system, which can be used e.g. for testing the effect of genetic alterations on the metastatic process of tumor cells, no animal test proposals are needed according to German law. Compared to other systems for monitoring intravasation, invasion and metastasis of tumor cells like matrigel assays the CAM assay offers superior comparability to the in vivo situation than pure in vitro assays.
Bilder & Videos
- In vivo simulation of intravasation, invasion and metastasis of tumor cells.
- In vivo test for determining the effect of drugs on intravasation, invasion and metastasis of tumor cells.
Rapid an reliable in vivo test with no need of animal test proposals
DKFZ and the University of Heidelberg offer this in vivo test as a service for industrial and academic institutions, for collaboration purposes and for licensing.
Publikationen & Verweise
- TaqMan-based quantification of invasive cells in the chick embryo metastasis assay. Van der Horst et al. 2004. See at: https://www.ncbi.nlm.nih.gov/pubmed/?term=Allgayer+Horst
- MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Asangani et al. 2008. See at: https://www.ncbi.nlm.nih.gov/pubmed/17968323
- Cetuximab attenuates metastasis and u-PAR expression in non-small cell lung cancer: u-PAR and E-cadherin are novel biomarkers of cetuximab sensitivity. Nikolova et al. 2009. See at: https://www.ncbi.nlm.nih.gov/pubmed/19276367
- Enzastaurin inhibits invasion and metastasis in lung cancer by diverse molecules. Körner et al. 2010. See at: https://www.ncbi.nlm.nih.gov/pubmed/20736951
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