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Kurzfassung

The here presented technology provides an apparatus for 4Pi STED which allows to generate a three-dimensional light intensity distribution comprising a very steep light intensity gradient between the areas of minimum and maximum light intensity.

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Hintergrund

In 4Pi STED scanning fluorescence light microscopy, it is known to use devices for forming threedimensional light intensity distribution comprising a spatially limited area of minimum light intensity that is enclosed by areas of higher light intensity of the fluorescence inhibiting light (Fig. 1a). The here presented technology provides an apparatus for 4Pi STED which allows to generate a three-dimensional light intensity distribution comprising a very steep light intensity gradient between the areas of minimum and maximum light intensity.

The apparatus comprises two objectives which are facing each other on a common optical axis, which focus light coming out of opposite directions into a common focal area, and which each have a pupil, and at least one light source. In case of three or more pairs of coherent light beams, the area of minimum light intensity is reduced to a small volume within the focal area. 

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Bilder & Videos

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Lösung

• Precise three-dimensional light intensity distribution
• Steep light intensity gradient

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Anwendungsbereiche

The presented technology allows generating an even steeper light intensity gradient between the area of minimum light intensity to the enclosing areas of higher light intensity than in isoSTED and can be used for 4Pi STED, RESOLFT or another variant of 4Pi GSD scanning fluorescence light microscopy.