Bio-based high-level production of the polymer building block glutarate (glutaric acid)
Metabolic engineered C. glutamicum with highly attractive glutarate titer, yield and productivity
There is a rapid growth of interest to use renewables for the manufacture of sustainable chemicals and materials. Glutarate, a carbon-5 dicarboxylic acid, is known as an attractive building block with recognized value for commercial plastics and other applications. Different approaches have demonstrated the bio-based-production of glutarate and its derivative 5-aminovalerate, however, glutarate titers have remained unattractively low so far.
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Bio-based high-level glutarate production with industrial attractive glutarate titer, yield and productivity.
Scientists of Saarland University established C. glutamicum as a production platform for the polymer building block glutaric acid through genome manipulation. They generated a strain with a highly attractive glutarate titer, yield and productivity. The new metabolic engineered C. glutamicum strain GTA-4 overexpresses - among other genes - a newly discovered 5-aminovalerate importer and forms glutarate from glucose in a yield of 0.27 mol mol−1. In a fed-batch process, GTA-4 produces more than 90 g L−1 glutarate from glucose and molasses based sugars in a yield of up to 0.70 mol mol−1 and a maximum productivity of 1.8 g L−1 h−1, while 5-aminovalerate is no longer secreted.
The bio-based glutaric acid can be purified to >99.9% purity. To demonstrate the potential of the bio-based glutaric acid as a polyamide precursor, nylon-6,5, a polyamide with unique structure, was successfully synthesized.
- bio-based production platform for the polymer building block glutaric acid
- 20-fold more product compared to other glutaric acid producers
- fully genome based; genetic stability without need for plasmids or antibiotic selection
- no side production of the derivate 5-aminovalerate
- use of other row materials than glucose should be feasible
We are looking for partners who are interested in the development and commercialization of the invention. Various licensing models are negotiable.
Universität des Saarlandes Wissens- und Technologietransfer GmbH
Dr. Nicole Comtesse
Universität des Saarlandes Wissens- und Technologietransfer GmbH Starterzentrum | Gebäude A1 1
- PCT PCT/EP2019/063559 anhängig
StichworteGlutaric acid, glutarate, bioplastic, bionylon, polymer, metabolic engineering, C. glutamicum, biotechnology Glutarsäure, Glutarat, Biokunststoff, Bionylon, Polymer, Pathwaydesign, C. glutamicum, Biotechnologie