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Novel heavy metal complexes as ex-vivo contrast agents for computed tomography scanning of biological samples

Ref-Nr: TA-B76184 / B76179


Kurzfassung

 

  • Novel contrast agents for histological methods
  • Drastic contrast enhancement for CT scanning
  • Facilitation of highly detailed 3D information


Hintergrund

One major limitation of conventional histological/pathological methods for diagnostic purposes in a clinical setting is the production of 2D images obtained by destructive preparation of 3D tissue samples. X-ray imaging techniques, such as micro computed tomography (µCT) allow for non-destructive 3D tissue imaging. However, for the visualization of morphological details in biological samples µCT is limited by the lack of contrast in soft tissue samples. Particularly, it is very challenging to obtain homogenous staining in large biological samples with conventional staining methods, due to diffusion problems.


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Lösung

Novel contrast agents for computed tomography scanning of biological samples allow for homogeneous tissue staining resulting in drastic contrast enhancement required for detailed 3D tissue imaging. Structural details such as cell nuclei are preserved and can be visualized using commercially available µCT setups at high resolution. The staining protocol produces superb contrast in 3D tissue samples without influencing conventional histological methods.


Anwendungsbereiche

COMMERCIAL OPPORTUNITIES

The innovation is applicable in the entire field of 3D histology and pathology with subsequent X-ray imaging. Potential applications a

  • Tumor diagnostics
  • Detection of 3D anatomy
  • Staining of tissue samples in agriculture and forestry


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