Characterization of Oocytes
System and Apparatus for the Characterization of Oocytes
Artificial fertilization of oocytes is an established method in human reproductive medi-cine that is routinely applied if couples desiring having children are not able to beget children on the natural way. Moreover, artificial fertilization is used for preserving and generating breeding and laboratory animals. The success of artificial fertilization deci-sively depends on the quality of the oocytes, in particular on the condition of the Zona pellucida (ZP), which is a gelatinous extracellular matrix layer surrounding the oocyte. During oocyte development, the ZP is initially hard, softens until the oocyte is fully mature and finally hardens again after fertilization, preventing the penetration of further sperm. The morphologic evaluation of the oocyte quality is usually estimated via microscopic inspection and is hence extremely subjective and strongly dependent on the individual observer. Other analysis tools are either risky and/or accompanied by ethical issues, meaning that to date there exists no reliable method to determine the time window for successful fertilization by evaluating the oocyte quality in an objective manner.
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A microsystem (see picture above) was developed to characterize the maturation pro-cess of oocytes in a time resolved and objective manner. The special design enables the gentle handling of oocytes by means of microfluidics. The use of electrodes ena-bles the detection of the oocyte’s hardening by electrical impedance spectroscopy. Therefore, the quality of the oocyte and its potential for fertilization and further devel-opment are evaluated in a non-destructive manner. Hence, the system can be used to assist artificial fertilization but might also be used for the development of contracep-tives.
• User-independent → objective analysis of the oocyte quality) and easy to use
• Enables substantial decision about oocyte quality
• Array design possible → enables simultaneous analyses of several oocytes
• Applicable for human and animal reproductive medicine
Reproductive Medicine, Artificial Fertilization, in-vitro Fertilization, In-tracytoplasmic Sperm Injection, in-vitro Diagnostics, Contraceptives Research
• Granted patent in Germany, international patent application pending.
• Functional model, successful proof of concept, ongoing research.
RWTH Aachen University is looking for partners for patent commercialization and/or for research partners for joint development and/or contract research.
StichworteReproductiveMedicine, Artificial Fertilization, Assisted Reproduction, In-vitro Fertilization, IVF, IntracytoplasmicSpermInjection, ICSI, In-vitro Diagnostics, Oocyte Maturation, Zona Pellucida