ACIT – A new tool for fermentation processes with non-model bacteria
Chromosomal integrating Cassette (ACIT) allowing indicible gene espression in alphaproteobacteria and non-model bacteria.
Biotechnological manufacturing of compounds and materials by fermentation is the most important way of transforming chemical manufacturing to sustainability.
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There are two major disadvantages of currently available fermentation processes: First, often feedstock material is needed which should preferably be used for food production (cf. for example the plate-tank issue with bio fuels). Second, many fermentation processes are performed with model bacteria (e.g. Escherichia coli), which are not naturally producing the desired product, so that it can hinder growth of the microorganism and reduce the yield of the product.
Both problems can easily be solved with the invention presented herein, being called ACIT ("Alphaproteobacteria chromosomally inserting transcription-control cassette"). The method allows to easily modify not only model bacteria, but also any type of non-model bacteria, so that for the production of a desired product a bacterium may be chosen which is already naturally producing (and therefore being adjusted to) the desired product.
The two major disadvantages of currently available fermentation methods, viability-problems of model bacteria if modified to produce non-native products and difficulties for using biological waste material as feedstock for fermentation processes can be solved with ACIT by either genetically modifying and applying non-model bacteria already adapted to the desired product (improving yield) or genetically modifying and applying non-model bacteria already able to digest biological waste material for fermentation.
The fields of application of ACIT are numerous due to the general applicability of the method for model bacteria as well as non-model bacteria, ranging from sustainable chemical manufacturing (compounds, polymers, fuels etc.) over production of pharmaceuticals to handling and using biological waste materials, for example from food industry.
On behalf of its shareholder Justus-Liebig-University Giessen TransMIT GmbH is looking for cooperation partners or licensees for further development in Germany, Europe, US/Canada, and Asia/Australia.
TransMIT Gesellschaft für Technologietransfer mbH
Kerkrader Str. 3
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StichworteSustainability, usage of waste streams, curdlan, polyhydroxybutyrate (PHB)